Vitreous body antigen was detected with the aid of specific rabbit anti bovine vitreous antibody and Staphyljococcus aureus Cowan 1 strain rich in protein A by coagglutination method. And the result was compared with the results obtained by the
usual
precipitation reaction-single immunodiffusion method, one dimensional double diffusion method, and two dimensional double diffusion method. The coagglutination method is based on the property of staphylococcal protein A to bind nonspecifically
the
Fc
fragment of Ig G of immune sera : upon addition of soluble antigen to these sensitized staphylococci, these reagent staphylococci with specific antibody bind to the soluble antigen through the Fab fragment of antibody and the lattice will be
formed
And
the study of the effect of protein A on hemagglutinin titers of rabbit anti-SRBC sera was attempted.
@ES The results obtained were as follows :
@EN 1. Coagglutination method was more easily performed than precipitation reaction and specific enough, and no special and expensive equipment with expert was needed to perform the test.
2. The titers of hemagglutinin and hemolysin of rabbit anti-SRBC sera were 1 : 6,400 and 1 : 25,600, respectively.
3. the titers of hemagglutinin and hemolysin of rabbit anti-SRBC sera treated with viable and formalinized Staphylococcus aurcus Cowan 1strain and 2-mercdaptoethanol respectively were decreased moderately.
4. the tiers of hemagglutinin and hemolysin of rabbit anti-SRBC sera treated with viable and formalinized Staphylococcus aureus Cowan 1 strain and 2-mercaptoethanol in turn were decreased markedly.
5. The concentration of Ig G of rabbit anti-SRBC serum treated with viable and formalinized Staphylococcus aureus Cowan 1 strain was markedly decreased, and serum Ig M was removed by 2-mercaptoethanol treatment. Above results suggested that
coagglutiation method presented may also be useful in the detection and identification of other low molecular weight, weak immunogenic, soluble antigens and hemagglutinin and hemolysin t rabbit anti-SRBC sera were Ig G and Ig M class.
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